Title Elucidating the relationship between hydration and ice recrystallization inhibition with c-linked antifreeze glycoproteins
Author Czechura, P.
Source 241p. . Availability: Univ. Microfilms, Ann Arbor, MI, United States. ISBN: 987-0-494-61362-7
Publication Date 2009
Notes In English. Ph.D. thesis. Ant. Acc. No: 91616. GeoRef Acc. No: 310323
Index Terms freezing; ice; Antarctica; Atlantic Ocean; Southern Ocean; Actinopterygii; antifreeze glycoproteins; biochemistry; Chordata; crystallization; hydration; living taxa; organic compounds; Osteichthyes; physiology; Pisces; proteins; synthesis; Teleostei; Vertebrata
Abstract Antifreeze glycoproteins (AFGPs) are a subclass of biological antifreezes isolated from Atlantic and Antarctic telost fish. At subzero temperature environments, these compounds have the ability to inhibit ice crystal growth and protect these organisms from cryoinjury and death. The native AFGPs show considerable promise as novel cryoprotectants, however, their limited bioavailability, cytotoxicity and inherent chemical and biological instability have precluded their widespread use. Consequentially, rationally design, non-toxic and stable AFGP analogues became an attractive synthetic challenge. While structural modifications made in previously synthesized C-linked AFGP analogues addressed these issues, further SAR studies were required to elucidate the key structural features necessary for potent inhibitors of ice recrystallization. The SAR studies described in this dissertation verify that hydration is related to ice recrystallization inhibition of the C-linked AFGP analogues. (mod. auth. abst.)
Publication Type monograph
Record ID 65007245