Title Differential gene expression of an Antarctic Chlorella in response to temperature stress
Author Chong, G.; Chu, W.; Othman, R.Y.; Phang, S.
Author Affil Chong, G., University of Malaysia, Institute of Biological Sciences, Kuala Lumpur, Malaysia. Other: International Medical University, Malaysia
Source Polar Biology, 34(5), p.637-645, . Publisher: Springer-Verlag, Berlin, Germany. ISSN: 0722-4060
Publication Date May 2011
Notes In English. 28 refs. Ant. Acc. No: 91539. GeoRef Acc. No: 309886
Index Terms algae; chlorophylls; ecology; plant ecology; temperature; Antarctica--Casey Station; Antarctica; Casey Station; chlorophyll; cold adaptation; DNA; genetics; organic compounds; pigments; Plantae; porphyrins; productivity; RNA; Wilkes Land
Abstract Changes in gene expression are an important response of Antarctic algae to temperature stress. The objective of this study was to investigate the differential gene expression of the Antarctic alga Chlorella UMACC 234 in response to temperature stress. The RNA was extracted from the cells grown at 4, 20, and 30C and converted to cDNA by reverse transcription. Differentially expressed genes (DEG) were isolated and identified using the GeneFishingTM DEG Kit (Seegene) with 20 arbitrary annealing control primers (ACP). The bands of interest were excised and purified from the agarose gel and then cloned and sequenced. A total of 22 DEG clones were isolated and identified, with 11 DEG detected only at 30C and six DEG detected only at 4C. Three DEG were detected at 4 and 20C while two were detected at 20 and 30C. The DEG were associated with functions such as photosynthesis, carbohydrate metabolism, electron transfer, and cell maintenance. Three DEG that showed high degree of similarity with sequences from the database were those code for Photosystem II P680 chlorophyll a apoprotein CP47 (PSII-CP47), aldose 1-epimerase, and a putative oxidoreductase. Real-time PCR analysis showed that the expression of the PSII-CP47 gene increased by threefold at 4C while that of the aldose 1-epimerase and oxidoreductase genes increased by threefold and eightfold, respectively, at 30C compared with 20C (optimal growth temperature).
URL http://hdl.handle.net/10.1007/s00300-010-0918-5
Publication Type journal article
Record ID 65006922