Title Antioxidant enzyme activity of filamentous fungi isolated from Livingston Island, Maritime Antarctica
Author Tosi, S.; Kostadinova, N.; Krumova, E.; Pashova, S.; Dishliiska, V.; Spassova, B.; Vassilev, S.; Angelova, M.
Author Affil Tosi, S., Universita di Pavia, Dipartimento di Ecologia del Territorio, Pavia, Italy. Other: Bulgarian Academy of Sciences, Stephan Angelov Institute of Microbiology, Bulgaria
Source Polar Biology, 33(9), p.1227-1237. Publisher: Springer-Verlag, Berlin, Germany. ISSN: 0722-4060
Publication Date Sept. 2010
Notes In English. 76 refs. GeoRef Acc. No: 309106. CRREL Acc. No: 65005998
Index Terms ecology; fungi; Antarctica--Livingston Island; Antarctica; biochemistry; enzymes; Livingston Island; organic compounds; proteins; Scotia Sea Islands; South Shetland Islands
Abstract From 18 soil samples taken in the vicinity of the permanent Bulgarian Antarctic base "St. Kliment Ohridski" (6238'29"S, 6021'53"W) on Livingston Island, 109 filamentous fungi were isolated on selective media. The most widespread fungal species were members of the genera Cladosporium, Geomyces, Penicillium and Aspergillus. Other species, already recorded in Antarctic environment, were also isolated: Lecanicillium muscarium, Epicoccum nigrum and Alternaria alternata. Thirty strains demonstrating good growth were screened for antioxidant enzymes superoxide dismutase (SOD) and catalase (CAT) that play an important role in the defense of aerobic organisms against oxidative stress, by converting reactive oxygen species into nontoxic molecules. Six of them showed high enzyme activity. The tested strains produced SOD with statistically significant higher activity at 15C than at 30C suggesting that this enzyme is cold-active. Such SOD could be useful in medicine and cosmetics. The best producer of cold-active SOD, Aspergillus glaucus 363, cultivated in bioreactors, demonstrated optimal growth temperature at 25C and maximum enzyme activities at 25 and 30C for SOD and CAT, respectively. The electrophoretical analysis showed that the fungus possesses Cu/Zn-SOD.
URL http://hdl.handle.net/10.1007/s00300-010-0812-1
Publication Type journal article
Record ID 91204