Title Haemagglutinin of the Antarctic seaweed Georgiella confluens (Reinsch) Kylin; isolation and partial characterization
Author Souza, B.W.S.; Andrade, F.K.; Teixeira, D.I.A.; Mansilla, A.; Freitas, A.L.P.
Author Affil Souza, B.W.S., Universidade Federal do Ceara-Fortaleza, Fortaleza, Brazil. Other: Universidade Federal do Rio Grando do Norte, Brazil; Universidad de Magallanes, Chile; Instituto de Ecología y Biodiversidad, Chile
Source Polar Biology, 33(10), p.1311-1318. Publisher: Springer-Verlag, Berlin, Germany. ISSN: 0722-4060
Publication Date Oct. 2010
Notes In English. 47 refs. GeoRef Acc. No: 309101. CRREL Acc. No: 65006002
Index Terms ecology; plant ecology; Antarctica-- King George Island; Antarctica; biochemistry; King George Island; nutrients; organic compounds; Plantae; proteins; sample preparation; Scotia Sea Islands; South Shetland Islands
Abstract The marine red alga Georgiella confluens collected from Mackellar Inlet, King George Island, South Shetland Islands, Antarctic, was used in the isolation of a protein with agglutinating activity. The Georgiella confluens haemagglutinin (GCH) was extracted with 20 mM phosphate buffer, pH 7.0, and purified through ion exchange chromatography, followed by affinity chromatography on immobilized porcine stomach mucin. Among the erythrocytes analysed (human A, B and O groups, rabbit and chicken), GCH agglutinated specifically chicken erythrocytes. Sodium dodecyl sulfate- polyacrylamide gel electrophoresis of the haemagglutinin revealed a single band of 21.5 kDa, while by gel filtration on Sephadex G- 100 its native molecular mass was 25.5 kDa, suggesting that GCH is a monomeric protein. Haemagglutination studies showed that the GCH activity was stable through temperature variations and did not exhibit divalent cation dependence. Furthermore, the haemagglutinin was inhibited by the complex glycoproteins of porcine stomach mucin and fetuin, whereas the mono-, di-, and trisaccharides tested showed no effect.
URL http://hdl.handle.net/10.1007/s00300-010-0818-8
Publication Type journal article
Record ID 91207