Title Antioxidant potential is positively correlated with mitochondrial enzyme activity in Antarctica and non-Antarctic notothenioid fishes
Author Crockett, E.L.
Author Affil Crockett, E.L., Ohio University, Deaprtment of Biological Sciences, Athens, OH
Source Polar Biology, 34(1), p.113-118. Publisher: Springer-Verlag, Berlin, Germany. ISSN: 0722-4060
Publication Date Jan. 2011
Notes In English. 43 refs. GeoRef Acc. No: 308831
Index Terms Antarctica; Southern Ocean; biochemistry; carboxylic acids; Chordata; ecology; enzymes; fatty acids; lipids; metabolism; nutrients; organic acids; organic compounds; Pisces; proteins; Vertebrata
Abstract Antarctic notothenioid fishes possess high oxidative capacities, large amounts of intracellular lipid combined with biological membranes enriched in polyunsaturated fatty acids, all of which could make these animals susceptible to oxidative injury, particularly in the form of lipid peroxidation. The central objective in this study was to examine capacities for oxidative metabolism and total antioxidant defense in Antarctic and non-Antarctic notothenioids in order to test the hypothesis that the cold-bodied Antarctic fishes possess elevated activities of citrate synthase (CS), matched by a more robust antioxidant (AOX) defense, than non- Antarctic species. CS activities and total AOX capacities were measured in brain and heart of 4 Antarctic species and 2 non- Antarctic species collected on the 2004 ICEFISH cruise. While no statistical differences are found among Antarctic and non- Antarctic fishes in either CS or AOX capacities, AOX capacity in both tissues expands with CS activity among individuals measured when all species are combined. There is also a 4.5-fold greater AOX capacity, when normalized to CS activity, in brain than in heart indicating the requirement for extra AOX defense in a tissue well known for its particularly high levels of phospholipids more prone to lipid peroxidation.
URL http://hdl.handle.net/10.1007/s00300-010-0864-2
Publication Type journal article
Record ID 91247